两种细菌粗多糖对草鱼的免疫调节作用

Two Types of Bacteria-polysaccharide on the Immune Regulation of Grass Carp

检测细菌多糖对草鱼的免疫调节作用。用酚水法分别从大肠杆菌和根癌农杆菌细胞壁中提取出粗多糖,注射草鱼,测定受试草鱼血液中白细胞数量,血清中抗体效价,肝脏、肾脏、脾脏、血清和粘液中过氧化物酶的活力。结果受试草鱼中的白细胞数量、血清抗体效价及过氧化物酶活力均具有明显提高。结论表明大肠杆菌和根癌农杆菌粗多糖对草鱼有免疫促进作用。

The purpose of detection of bacterial-polysaccharide on the immune regulation on grass carpo We can use phenol water method to get polySaccharides from E. coli and Agrobacterium tumefaciens cell wall Injec- tion of grass carpdetermination the number of leukocyte of blood ,the vitality of peroxidase in Liver, kidney, spleen, serum and mucus The results of subjects are the number of grass carp in the leukocyte, serum antibody titer and peroxidase activity have markedly improved Conclusion is E.coli and Agrobacterium tumefaciens coarse polysaccharide have strong immune on grass carp.