新生鼠缺氧缺血性脑损伤早期闪光视觉诱发电位和血清神经元特异性烯醇化酶浓度改变监测

Significance of F-VEP waves and serum NSE levels in monitoring of early hypoxic ischemic brain damage in neonatal rats

目的:探讨新生大鼠缺氧缺血性脑损伤(HIBD)发生后0、6、12、24、48h早期闪光视觉诱发电位(F-VEP)和血清神经元特异性烯醇化酶(NSE)浓度改变。方法:SD新生大鼠80只,随机分为假手术组和HIBD组,每组均40只,按HIBD动物模型建立后0、6、12、24、48h对应的时段每组每时段各8只,采用闪光刺激法检测各组大鼠视觉诱发电位,采用免疫放射分析法(RIMA)检测血清NSE浓度。结果:HIBD组0、6、12、24、48h各时段F-VEP潜伏期较假手术组均明显延长(P均<0.001),各组波幅明显降低(P均<0.001);HIBD组血清NSE浓度在0、6、12h与假手术组比较差异均无显著性,24h比假手术组明显升高(P<0.001)。结论:F-VEP有助于早期监测HIBD的发生。

Objective：To investigate the changes of flash visual evoked potentials（F-VEP） waves and serum neuron specific enolase （NSE） levels in neonatal rats with early hypoxic ischemic brain damage （HIBD） and to evaluate the significance of F-VEP monitoring. Methods：Eighty neonatal rats were randomly divided into the sham operation group and the HIBD group,40 rats each group. They were tested with F-VEP at different times （0,6,12,24,48 h） after the model of HIBD was established. Serum NSE levels were tested with immunoradiometric assay.Results：In comparison with the sham operation group, the F-VEP waves latencies in HIBD group were longer at 0,6, 12,24,48 h, respectively （t=6.90,P〈0.001 ;t=7.24,P〈0.001 ;t=16.85,P〈0.001 ;t=18.56,P〈0.001 ;t=16.00,P〈0.001）. The waves amplitudes in HIBD group were lower than those in the sham operation group at 0,6,12,24,48 h,respectively（t=4.73 ,P〈0.001 ;t=5.47 ,P〈 0.001 ;t=5.43,P〈0.001 ;t=9.19,P〈0.001 ;t=8.12,P〈0.001）. There was no significant difference in serum NSE levels in both group at 0,6,12 h. Serum NSE levels in HIBD group were significantly increased at 24h（t=24.21 ,P〈0.001）.Conclusion ：F-VEP would help to improve the level of HIBD monitoring.