结核性胸腔积液病人IL-12、IL-18、TNF-α的水平及变化

Study of level and change of IL-12、IL-18 and TNF-α in tuberculous pleural effusion

目的:探讨三种与结核病(TB)保护性免疫有关的细胞因子白介素-12(IL-12)、白介素-18(IL-18)和肿瘤坏死因子-α(TNF-α)在结核病(TB)免疫发病机制中的作用,为设计新的疫苗和开展辅助性免疫疗法提供依据。方法:检测39例结核性胸腔积液病人胸水中和血清中IL-12、IL-18、TNF-α的水平;并对治疗好转过程中10例Ⅲ型病人和5例Ⅳ型病人这些细胞因子在胸水中的变化进行观测。结果:除IL-18外,其他两种细胞因子胸水中与血清中含量均有显著性差异;IL-12与TNF-α之间有相关关系。治疗好转过程中,Ⅲ型病人绝大多数见IL-12、IL-18水平增高。结论:IL-18用于疫苗佐剂应该优于IL-12;复治组病人IL-12与TNF-α之间呈显著正相关,开展免疫疗法时可参考。

Objective：To study the role of three kinds of protective cytokine IL--12,IL-18 and TNF-α in the immunopathogenesis of tuberculosis for designing new vaccine and developing immunotherapy.Methods ：The levels of IL-12, IL-18 and TNF--α were measured by sandwich ABC-ELISA and sandwich double antibody ELISA kits in 39 cases of tuberculosis pleural effusion.Significance test and correlation analysis were undertaken.The changes of these kinds of eytokiness in plural fluid （PF） were observed during the course from progression to resolving stage showed by X-ray in 10 cases of type m and 5 cases of type Ⅳ.Results：Except IL-18,the concentration of other two eytokines in PF and serum had significant difference.Only IL-12 and TNF-α displayed correlation relationship.The majority of patients had the increasing levels of IL-12 and IL-18. Conclusion：IL-18 should be superior to IL-12 as adjuvant agent of vaeeine.IL-12 and TNF-α show significantly positive correlation in the retreatment patients,which could be used as reference when developing immunotherapy.