MCF-7乳腺癌细胞培养上清对树突状细胞抑制作用的研究

Effects of soluble factors secreted by MCF-7 breast cancer cells on dendritic cells

目的:应用MCF-7乳腺癌细胞分泌的上清液培养正常外周血树突状细胞,探讨MCF-7乳腺癌细胞分泌因子对正常树突状细胞分化、成熟及功能的影响。方法:应用MCF-7乳腺癌细胞的培养上清和GM-CSF、IL-4及TNF-α培养正常外周血单个核细胞,检测所诱导的树突状细胞(DC)及其致敏的CTL活性。结果:MCF-7乳腺癌细胞培养上清能够明显抑制正常树突状细胞的分化成熟及抗原提呈能力,CD80、CD83、CD86和HLA-DR的表达明显降低,与正常对照差异显著(P<0.01);CTL对MCF-7细胞杀伤活性为17.35%与对照组56.14%比较差异显著(P<0.01);IL-12分泌和共刺激T淋巴细胞所分泌的IFN-γ明显降低(P<0.01)。结论:MCF-7乳腺癌细胞上清明显抑制所共培养的树突状细胞的分化、成熟及抗原提呈能力。

Objective： To investigate the effects of soluble factors secreted by MCF-7 breast cancer cells on the differentiation,maturalion and function of DCs. Methods： Mononuclear cells were cultured with the culture supematants from primary MCF-7 cells. Combination of granulocyte-macrophage colony stimulating factor（ GM-CSF）, interleukin 4（IL-4） and tumor necrosis factor-a（TNF-a） was used to stimulate the cultured mononuclear cells. Then DCs and CTL assays were analyzed. Results： MCF-7 cell supernatant-treated DCs resulted in low expression of CD80, CD83, CD86 and HLA-DR, and the inhibition rate of CTL was 17.35% significantly lower than 56.14% induced by the control DCs （ P 〈 0.01） .IL-12 secreted by DCs and IFN-γproduced by PBMNC were all significantly lower than those in the control group （ P 〈 0.01）. Condusion： Experiments in vitro shows that the culture supernatants from MCF-7 breast cancer cells could inhibit the development and functions of DCs.