重症肌无力中白介素4、15、18与端粒酶活性的相关性研究

The correlation between interleukin-4,15 as well as 18 and telomerase activity in myasthenia gravis

目的:探讨IL-4、IL-15、IL-18在重症肌无力(MG)中的表达及其与CD4+T淋巴细胞端粒酶活性的相关关系。方法:应用ELISA及重复扩增-酶联免疫吸附实验(PCR-ELISA)检测30例MG患者(MG组)和30例健康对照者(NC组)血清中的IL-4、IL-15、IL-18水平和CD4+T淋巴细胞端粒酶活性。结果:MG组血清IL-4、IL-15、IL-18水平及CD4+T淋巴细胞端粒酶活性显著高于NC组,差异具有统计学意义(P<0.01);MG组CD4+T淋巴细胞端粒酶活性与血清IL-4、IL-15、IL-18水平均存在正相关关系(P<0.01)。结论:IL-4、IL-15、IL-18及CD4+T淋巴细胞端粒酶均参与了MG的发病过程;MG中IL-4、IL-15及IL-18有可能通过各种途径直接或间接的上调CD4+T淋巴细胞的端粒酶的活性。

Objective： To detect the serum levels of IL-4, IL-15 and IL-18 in myasthenia gravis（MG） and investigate the correlations between telomerase activity of CD4＋ T lymphocytes and these interleukins. Methods： The serum levels of IL-4, IL-15 and IL-18 were detected with ELISA and telomerase activity was detected by polymerase chain reaction enzyme-linked immonosorbent assay（PCR-ELISA） in 30 MG pa- tients and 30 negative controls. Results： The levels of IL-4, IL-15, IL- 18 and telomerase of CD4＋ T lymphocytes in MG were significantly higher than those in negative controls （P 〈 0.01 ）. There were positive correlations between the serum levels of IL-4, IL-15, IL-18 and telomerase activity of CD4＋ ＋ T cell in MG patients （P 〈 0.01 ）. Conclusion： IL-4, IL-15, IL-18 and telomerase activity of CD4 ＋ T lymphocytes are involved in the pathogenesis of MG; IL-4, IL-15 and IL-18 may upregulate the telomerase activity of CD4＋ T cells.