bcl-2及p53癌基因在子宫内膜癌中的表达及意义

RELATIONSHIP BETWEEN THE EXPRESSION OF bcl-2 AND p53 PROTEINS AND THE BIOLOGIC BEHAVIORS OF HUMAN ENDOMETRIAL CARCINOMA

目的探讨子宫内膜癌及其癌前病变ｂｃｌ－２及ｐ５３基因的表达及与凋亡的关系。方法利用微波免疫组化ＡＢＣ方法检测肿瘤组织内ｂｃｌ－２蛋白及ｐ５３蛋白的分布情况。结果正常和单纯性增生子宫内膜ｂｃｌ－２蛋白和ｐ５３蛋白均为阴性反应。ｂｃｌ－２蛋白和ｐ５３蛋白阳性率在子宫内膜癌分别为５１．９％和４８．１％；在癌前期病变分别为３３．３％和１６．７％，均明显高于非肿瘤子宫内膜（Ｐ＜０．０５）。ｂｃｌ－２蛋白的表达强度随子宫内膜癌分化程度的增加而增加。ｐ５３蛋白则与肿瘤细胞的浸润和临床分期有关（Ｐ＜０．０５）。结论子宫内膜癌的发生、发展中细胞凋亡起着重要作用。ｂｃｌ－２蛋白是子宫内膜癌的早期指标。而突变型ｐ５３蛋白则与子宫内膜癌的浸润、转移及不良预后有关。

Objective To investigate the relationship between the expression of bcl-2 and p53 proteins and apoptosis in human endometrial carcinoma and its precancerous lesions.Method The localization of bcl-2 and p53 proteins in tumour tissues were observed by using microwave-immunohistochemistry.Results The expression of bcl-2 and p53 proteins were negative in normal and proliferated endometrial mucosa.The incidence of bcl-2 and p53 proteins in endometrial carcinoma were 51.9% and 48.1% respectively and those in its precancerous lesions were 33.3% and 16.7 % respectively .there were significant differences compared with normal endometrial mucosa (P<0.05).The expression of bcl-2 protein in human endometrial carcinoma was related to differentiated degree of cancer (P<0.05),and that of p53 protein was related to the depth of cancer infiltration and clinic stages (P<0.05).Conclusion Cell apoptosis plays an important role in the carcinogenesis of uterine endometrial mucosa .The detection of bcl-2 protein is useful for earlier diagnosis of endometrial carcinoma and the expression of mutation-type p53 is related to the infiltration,metastasis and poor prognosis of endometrial carcinoma.