清络通痹颗粒对类风湿关节炎患者成纤维样滑膜细胞增殖及RANKL表达的影响

Effects of Qingluo Tongbi Granula on Proliferation of Fibroblast-like Synoviocytes and RANKL Expression in Rheumatoid Arthritis Patients

目的观察清络通痹颗粒(QLT)含药血清对类风湿关节炎(RA)患者成纤维样滑膜细胞(FLS)增殖及核因子κB受体活化子配体(RANKL)表达的影响。方法取3～5代FLS,MTT比色法检测含药血清对FLS增殖的影响,RT-PCR检测含药血清对FLSRANKL表达的影响。结果QLT大剂量含药血清作用72h,RA患者FLS增殖受到抑制,对RANKL表达有抑制作用。结论QLT大剂量含药血清可抑制RA患者FLS的异常增殖,并下调RANKL的表达,这可能是清络通痹颗粒治疗RA的重要机制之一。

Objective To observe the effect of serum containing Qingluo Tongbi Granula （QTG） on proliferation of fibrob- last - like synoviocytes （FLS） and receptor activator of nuclear factor - κB ligand （RANKL） expression in FLS from patients with rheumatoid arthritis. Methods Synovial specimens obtained from the joint of patients were minced into small pieces for the culture of FLS. The morphological features of FLS subcultrued for 3 - 5 generations were observed under inverted microscope and electron microscope. The effect of QTG - containing serum on the proliferation of FLS was examined by MTT assay. The expression of RANKL in FLS was assessed by RT- PCR. Results After cocultured by serum containing large - dose QTG for 72 hours, proliferation of FLS and expression of RANKL were inhibited. Conclusion QTG can inhibit FLS proliferation, and reduce the expression of RANKL, which may be one of the therapeutic mechanisms of QTG for rheumatoid arthritis.