蜂胶醇提物对扑热息痛致小鼠急性肝损伤的保护作用

Hepatoprotective Effect of Alcohol-Extract Propolis Against Acetaminophen-induced Acute Hepatic Injury in Mice

目的研究蜂胶醇提物对扑热息痛(Paracetamol,APAP)致小鼠急性肝损伤的保护作用并探讨其可能的作用机制。方法取C57BL/6MT(-/-)小鼠63只,随机分为9组,每组7只,各组于每天上午9点至10点称重,正常对照组和模型组给予NS20mL·kg-1,蜂胶醇提物组分别给予12.5,25,50和100mg·kg-1蜂胶醇提物,20%乙醇+A-PAP组和20%乙醇对照组给予20mL·kg-1乙醇,蜂胶醇提物对照组给予100mg·kg-1蜂胶醇提物,均为灌胃给药,每天1次,连续4d;最后1次给药30min后,除正常对照组,蜂胶醇提物对照组和20%乙醇对照组scNS10mL·kg-1之外,其余各组均scAPAP380mg·kg-1制备小鼠急性肝损伤模型。检测小鼠血清ALT、AST和LDH的活性;观察肝脏组织病理变化情况和肝体比;测定肝组织匀浆GSH,GSSG和MDA的含量。结果与APAP模型组相比,蜂胶醇提物可以明显降低小鼠血清中ALT、AST和LDH的活性,降低肝组织中MDA的含量,升高GSH、GSH/GSSG比值,肝组织病理损伤明显减轻;蜂胶醇提物对照组GSH的含量与GSH/GSSG比值升高;而20%乙醇对照组对上述指标均无明显影响。结论蜂胶醇提物对APAP致小鼠急性肝损伤具有明显的保护作用。

Objective To investigate the hepatoprotective effect of Propolis alcohol - extract （PAE） against acetaminophen （APAP） - induced acute hepatic damage in mice and to explore the possible mechanism. Methods Sixty - three C57BL/6 MT（ - / - ）mice were equally randomized into 9 groups. The normal control group and the model group received gastric gavage of normal saline （20 mL ·kg^-1） , four PAE groups were given PAE in the dose of 12. 5, 25, 50 and 100 mg ·kg^-1 respectively, alcohol ＋ APAP group and alcohol control group received 20 % alcohol 20 mL·kg^-1 and PAE control group was given PAE in the dose of 100 mg·kg^-1 qd, for 4 consective days. Thirty miniutes after last administration, the mice in the normal control group, PAE control group and alcohol control group received saline 10 mL·kg^-1, and the mice in other groups received APAP 380 mg·kg^-1 to induce acute hepatic injury. The activities of serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and lactate dehydrogenase （LDH） were determined, and the liver tissues were collected for histopathological assessment by HE staining under light microscope. The ratio of glutathione （GSH） and oxidized glutathione （GSSG）, and the content of GSH, GSSG and malondialdehyde （MDA） in liver homogenate were also measured. Results Compared with the model group, PAE could markedly decrease serum ALT, AST and LDH activity, reduce the MDA level in liver homogenate, and increase hepatic GSH content and the ratio of GSH/GSSG in the liver ho- mogenate. The hepatic histopathological changes in liver were also significantly ameliorated. In PAE control group, GSH content and the ratio of GSH/GSSG were also increased. However, the above indexes remained unchanged in alcohol control group. Conclusion The propolis alcohol - extract can prevent the liver from PAP - induced acute hepatic injury.