不同产地火炭母中槲皮素的含量测定方法研究

Content Determination of Quercetin in Polygonum chinense L.from Different Growing Areas

目的建立火炭母药材中槲皮素的的含量测定方法,测定10个产地火炭母中槲皮素的含量。方法采用RP-HPLC测定药材中槲皮素含量,色谱柱:AgilentTC-C18色谱柱(4.6mm×250mm,5μm);流动相:甲醇-0.6%磷酸(52:48)等度洗脱;流速:1mL·min-1;柱温:(25±1)℃;检测波长:365nm;洗脱时间:20min;进样量:20μL。结果槲皮素标准曲线回归方程为:Y=85355X-48.16(r=0.9993),在0.004～0.012mg·mL-1浓度范围内线性关系良好,平均回收率为100.3%,RSD=1.44%,不同产地火炭母中槲皮素的含量范围为183.0～848.1μg·g-1。结论所用方法简便、准确,可用于火炭母药材的质量控制;相同季节、不同产地火炭母中槲皮素的含量差异较大。

Objective To determine quercetin content in Polygonum chinense L. from different growing areas. Methods RP- HPLC was used to determine quercetin content. Chromatographic conditions were as follows： chromatographic col- umn of Agilent TC - C18 （4.6 mm × 250 mm, 5 μm）, mobile phase consisting of methanol - 0.6 % phosphoric acid （52： 48）, with a velocity of 1 mL ·min^-1, column temperature at （25 ± 1）℃, detection wavelength being 365 nm, elution time of 20min, and an injection volume of 20 μL. Results Standard curve regression equation of quercetin is： Y= 85 355X - 48.16（ r = 0. 999 3）. A good linearity was showed in the range of 0. 004 - 0. 012 mg · mL^-1, with average recovery being 100.3 % , and RSD of 1.44 %. The content of quercetin in the samples from different growing areas is in the range of 183.0 - 848.1 μg· g^- 1. Conclusions The method is simple and reliable, and can be used to control the quality of Polygonum chinense L. The existence of some differences of quercetin content between medicinal materials from different growing areas indicates that we should make a quality standard to ensure the safety and effectiveness of Polygonum chinense L. in clinic.