正交试验优选枳实中新橙皮苷的提取工艺

Optimization of Extraction Technology for Neohesperidin in Fructus Aurantii Immaturus by Orthogonal Experiment

目的优选枳实中新橙皮苷的最佳提取工艺。方法采用正交试验,以新橙皮苷含量为指标,考察溶剂浓度、溶剂用量、回流时间、回流次数对枳实中新橙皮苷提取工艺的影响。结果提取次数为影响枳实中新橙皮苷提取效果的主要因素。确定提取工艺为6倍量的70%乙醇提取3次,每次1h。结论优选的提取工艺稳定,提取率高。

Objective To optimize the extraction process of neohesperidin in Fructus Aurantii Immaturus. Methods The optimum proess was investigated by L9（34） orthogonal design with the content of neohesperidin as the observation index. Results Extraction times were the main factors for neohesperidin extraction. The optimal process condition was as follows： adding 6 -fold 70 % ethanol, extracting for 3 times, one hour for each extraction. Conclusion The optimal extraction technology of neohesperidin in Fructus Aurantii Immaturus is stable and reliable.