丹参成分对四氯化碳损伤原代培养大鼠肝细胞的作用

Effect of Components in Salvia miltiorrhiza on Carbon Tetrachioride Induced Cytotoxicity in Primarily Cultured Rat Hepatocytes

目的：研究丹参成分对四氯化碳（CCl4）损伤原代培养大鼠肝细胞的作用。方法：CCl4致体外原代培养大鼠肝细胞损伤模型，观察肝细胞的ALT活性及超微结构。结果：丹参的乙酸乙酯萃取物（EAE）和正丁醇萃取物（nBE）均可使10mmol／LCCl4损伤的原代培养大鼠肝细胞培养液中的ALT活性显著降低。从乙酸乙酯萃取物中分离出A、B、C、D4种组分；在1．0mg／ml时C、D组分有显著护肝作用。含量较多的D组分经小鼠CC14肝损整体模型验证，亦有显著的护肝作用。D组分经鉴定为丹参的酚酸性成分。结论：丹参的酚酸性成分为主要护肝部分。

ObjectiveTo study the effect of components in Salvia miltitorrhiza Bunge(SmB) on carbon tetrachloride-induced cytotoxicity in primarily cultured rat hepatocytes. Methods:The ALT level was measured andthe cell ultrastructure observed on the model of carbon tetrachloride-induced cytotoxicity in primarily cultured rathepatocytes. Results:Both ethyl acetate and n-butanol extracts of SmB significantly lowered the ALT level in thecultured media exposed to 10 mmol/L. CCl4. The constituents A .B,C and D were isolated from the ethyl acetateextract of SmB. The constituents C and D at the dosage of 1. 0 mg / ml were proved to have highly antihepatoto xic effect. The effect of constituent D on carbon tetrachloride-induced hepatotoxicity in mice were proved. Theconstituent D was identified as phenolic content including protocatechnic aldehyde etc. of SmB. Conclusion: Thephenolic content of SmB was proved to have highly antihepatotoxic effect.