基因重组人三叶因子2对实验性大鼠胃溃疡的疗效及其作用机制

Effect of hTFF2 gene therapy on the healing of experimental gastric ulcer in rats and its mechanism

目的探讨人三叶因子2对实验性大鼠胃溃疡愈合的影响及其机制。方法将48只Wistar大鼠随机分为治疗组和对照组。应用冰乙酸法制作大鼠慢性胃溃疡模型，治疗组24只应用pcDNA3．1-hTFF2100μg／只，在造模时经胃壁黏膜下注入，并每日肌肉注射生理盐水100μl／只至标本采集。对照组24只应用等容积pcDNA3．1100μg／只，在造模时经胃壁黏膜下注入，并每日肌肉注射生理盐水100μl至标本采集。术后第3天、第7天、第14天各处死治疗组和对照组各8只大鼠。测定治疗组和对照组胃溃疡指数、溃疡旁胃黏膜内PCNA阳性细胞增值指数及溃疡底部血管生成的变化。结果第3天，治疗组与对照组的上述各项指标无显著性差异（P〈0．05）。第7天、第14天治疗组较对照组的溃疡旁胃黏膜内PCNA阳性细胞增值指数及溃疡底部新生血管明显升高，两者相比差异有显著性（P〈0．05），而其中胃溃疡指数在第14天治疗组较对照组显著缩小，差异具有统计学意义（P〈0．01）。结论hTFF2对实验性大鼠胃溃疡愈合有促进作用，促进胃黏膜上皮细胞增殖和溃疡底部血管生成是其促进溃疡愈合的机制之一。

Objective To study the effect of hTFF2 gene therapy on the healing of experimental gastric ulcer in rats and its mechanism. Methods 48 wistar rats were randomly divided into therapy group and control group. Gastric ulcer was induced in rats by the glacial acetic acid method. And the site around the ulcer of each rat in therapy group was injected with peDNA3.1 - hTFF2 100 p,g. The same volume of pcDNA 3. 1 was used in control group. Each group was injected into 100 μl NS through intramuscular injection until executed. Rats in therapy group and control group were executed separately on the 3rd day, the 7th day and the 14th day after the operation （8 rats in each group at each time point）. Results 14 days after ulcer inducement, the ulcer area was respectively （ 17.03±2.2） mm2 and （9.58±3.12 ）mm2 in control group and therapy group （P = 0. 002）. On the 7th and 14th day after ulcer inducement, the PCNA LI and microvessel in the granulation at the ulcer base in therapy group are higher than in control group, there was significant difference between the two groups （ P 〈 0. 005 ）. Conclusion pcDNA3. 1 - hTFF2 gene therapy can promote the healing of gastric ulcer in experimental rats which through promoting gastric epithelial cell proliferation and angiogenesis at the ulcer base.