摘要
缺血是继发性脊髓损伤(SCI)的重要机制之一,而内皮素(ET)是迄今所知作用最强、持续最久的缩血管多肽,并且ET也是一种能够在中枢神经系统中表达的神经递质。本实验通过观察SCI后脊髓组织和血浆中ET含量的变化,研究ET与急性SCI的关系,并探讨其可能的发生机制。方法:应用改良Alen氏脊髓损伤模型,选用雄性SD大鼠40只,造成5g×10cm强度的SCI。应用放射免疫法(RIA)测定SCI后6小时、24小时及14天时脊髓组织和血浆中ET的浓度。分别在术前、SCI后及处死前记录MEP。结果:损伤后血浆中ET浓度无明显改变,而脊髓组织中的ET含量进行性升高。6小时上升至对照组的113%,24小时上升至124%,14天上升至139%。结论:SCI后脊髓组织中ET含量迅速升高并且持续较长时间。
Ischemia is one of the most important mechanisms of secondary spinal cord injury (SCI).Endothelin (ET) is the most potent and long-lasting vasoconstrictive peptide,which is expressed in the central nervous system and may act as a neuromodulator.ET has been proved to play an important role in some cerebral vascular diseases,and there were also experimental evidence indicating the possible relationship between ET and SCI.In this study,the ET level in both spinal cord and plasma was measured after an acute experimenta lSCI in order to explain the relation between ET and acute SCI and to explore the possible origination of ET.The experimental acute SCI model was created by modified Allen’smethod. At 6h, 24h and 14d after SCI,ET level in spinal cord and plasma were measured by radioimmunoassay. After SCI, there was littlevariation of the concentrations of ET inplasma, while in the spinal cord the concentrations were elevated progressively during 14 days,from 113% at 6h to 139%in 14d.ET seems to play an important role in SCI as its level increased rapidly and lasted for long time after SCI.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1998年第10期594-597,共4页
Chinese Journal of Orthopaedics
关键词
脊髓损伤
血浆
内皮素
缺血
Spinal cord injuries Immunization Plasma