摘要
卵母细胞冷冻保存具有广泛而潜在的应用价值。文章主要分析了不同冷冻方法对绵羊GV期卵母细胞发育效果的影响。GV期卵母细胞程序化冷冻解冻后形态正常率(54.8%)和体外培养成熟率(14.7%)均显著或极显著低于细管玻璃化冷冻(71.8%,29.5%;P<0.05)和OPS玻璃化冷冻(78.3%,P<0.01;35.4%,P<0.05);3种不同方法冷冻GV期卵母细胞成熟率与对照组相比均差异极显著(P<0.01)。
Oocytes cryopreservation is a potentially valuable technique for conservation of animal genetic resources. In this experiment, the influences of freezing methods on developmental capability of ovine GV stage oocytes were examined. Oocytes were frozen by pro grammed freezing,straw vitrification and open pulled straw vitrification （OPS） method, fresh oocytes served as the control. The results showed that, the rates of morphologically normal oocytes（54.8% ） and the maturation rate（14.7% ） with programmed freezing method were lower than straw（71.8 %, 29.5 % %; P〈0. 05） and OPS vitrification method（78.30% , P〈0.01 ; 35.4 %, P〈0. 05）, and the control group was the highest （P〈0. 01）.
出处
《中国草食动物》
2009年第6期3-4,共2页
China Herbivores
关键词
GV期卵母细胞
冷冻
发育能力
绵羊
GV stage oocytes
freezing
developmental capacity
ovine
