摘要
目的观察银杏叶提取物(EGB)对小鼠缺氧及缺氧性记忆障碍的影响。方法采用NaNO2、KCN及断头等方法造成小鼠缺氧致死模型。以NaNO2造成小鼠缺氧性记忆缺失模型,并用跳台法作行为检测,TBA法测定丙二醛(MDA)含量,Green法测定NO含量。结果EGB(40,80mg·kg-1,ig,qd×14天)可使以上模型缺氧小鼠的存活时间延长;减少记忆缺失小鼠的跳台错误次数,延长其SDL,并使脑组织中升高的MDA和NO含量降低。结论EGB有明显的耐缺氧作用;对NaNO2引起的缺氧性记忆障碍有明显改善,此作用可能与脑组织MDA、NO含量的变化有一定关联。
Objective To investigate the influence of extracts of ginkgo biloba leaves (EGB) on anoxia and impairment of memory induced by anoxia in mice. Methods The anoxic models induced by NaNO 2, KCN and cutting head were used. The anoxic impairment of memory was induced by NaNO 2. The ability of memory was explored using step down test. TBA and Green methods were respectively adopted to measure malondialdehyde (MDA) and nitric oxide (NO).Results EGB(40,80 mg·kg -1 , ig, qd×14days) markedly prolonged the survival time in anoxic mice, reduced the mistaken time of step down and prolonged SDL, reduced the MDA and NO contents in the cerebrum for the mice of impairent of memory. Conclusions EGB has protective effect against anoxia. EGB improves the anoxic impairment of memory induced by NaNO 2. The effects of EGB are probably related to the change of MDA and NO contents.
出处
《安徽医科大学学报》
CAS
1998年第5期351-353,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金
关键词
银杏叶提取物
缺氧
记忆障碍
小鼠
extracts of ginkgo biloba leaves
anoxia
memory
malondialdehyde
nitric oxide