摘要
目的：了解幽门螺杆菌（Ｈｐ）对胃上皮细胞分泌ＩＬ－８的影响。方法：将Ｈｐ与胃上皮细胞共同孵育２４ｈ，ＥＬＩＳＡ法检测上清液中ＩＬ－８的浓度。结果：Ｈｐ可直接诱导胃上皮细胞分泌ＩＬ－８；热灭活、甲醛溶液固定后的菌株该作用明显减弱（Ｐ＜０．０１）；含有细胞毒素相关基因Ａ（ｃａｇＡ）株的诱导作用大于不含ｃａｇＡ株，差异明显（Ｐ＜０．０５）。结论：Ｈｐ能够刺激胃上皮细胞分泌ＩＬ－８；
Objective: To study the effects of Helicobacter pylori (Hp) on IL 8 protein secretion in established gastric epithelial cell lines. Methods: Human gastric epithelial cell lines (MKN45,BGC823,SGC7901) were co cultured with Hp CCUG17874,G50 and 5 clinical isolates (three cagA positive and two cagA negative). IL 8 was measured by enzyme linked immunosorbent assay. Results: Hp induced IL 8 secretion from the three cell lines. IL 8 secretion was reduced by heat killing, formalin fixation of Hp (P<0 01). CagA positive strains induced significantly higher IL 8 secretion than negative strains (P<0 05). Conclusion: Hp directly increases gastric epithelial IL 8 protein expression, and it is related to the cagA gene.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第5期452-454,共3页
Academic Journal of Second Military Medical University
