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TAT-Hsp70融合蛋白表达载体的构建及表达

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摘要 为了得到Hsp70与TAT-PTD(protein transduction domain)的融合蛋白,利用PCR的方法先将9个TAT基因序列置于Hsp70基因序列之前,再克隆到pEASY-E1载体上。pEASY-E1/TAT-Hsp70重组子在大肠杆菌Transetta(DE3)内诱导表达,纯化后经Western blot鉴定,证明其是具有生物学活性的目的蛋白。试验成功得到了可溶性TAT-Hsp70融合蛋白,为应用蛋白转导技术进一步研究Hsp70对细胞的热保护机制奠定了基础。
作者 王恺 田文儒 高善颂 姜斐斐 王小伟
机构地区 青岛农业大学动物科技学院
出处 《上海畜牧兽医通讯》 2009年第6期15-17,共3页 Shanghai Journal of Animal Husbandry and Veterinary Medicine
基金 山东省自然科学基金(Y2003D03) 国家自然科学基金(30871893)
关键词 热休克蛋白 TAT蛋白 原核表达
分类号 Q78 [生物学—分子生物学] Q782 [生物学—分子生物学]
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参考文献13

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上海畜牧兽医通讯
2009年 第6期

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