非小细胞肺癌中Kiss-1的表达和淋巴管生成的意义

Significance of Kiss-1 Expression and Lymphangiogenesis with Non-Small Cell Lung Cancer

目的探讨非小细胞肺癌(Non-Small Cell Lung Cancer,NSCLC)转移抑制基因Kiss-1表达下调或缺失和淋巴管生成与淋巴结转移是否具有协同作用。方法兔抗人单克隆抗体Kiss-1蛋白metastin(45-54)-NH2和抗Podoplanin单克隆抗体(Elivision法)对68例NSCLC术后石蜡切片进行染色,显微镜下观察Kiss-1的表达和肿瘤间质及癌旁组织淋巴管生成情况。结果①NSCLC组织内淋巴结阳性组Kiss-1的表达阳性率为39.4%(13/33),淋巴结阴性组Kiss-1的表达阳性率为91.4%(32/35),两组相比差异有统计学意义(P<0.05);②肿瘤组织间质中淋巴结阳性患者淋巴管密度(Lymphatic vessel density,LVD)平均值为15.73±0.74,淋巴结阴性患者LVD平均值为8.31±0.32,二者相比差异有统计学意义(P<0.05)。③logistic回归分析显示,仅LVD对淋巴结转移有影响(P<0.05),其相对危险度(RR)为2.065,95%可信区间(1.202,3.547)。而Kiss-1的表达下调无意义,P值为0.569,95%可信区间为(0.011,12.143)。二者之间对淋巴结转移无明显协同作用(P<0.05)。结论转移抑制基因Kiss-1的表达下调和淋巴管生成对淋巴结转移未见明显协同作用。

Objective To study the synergy between down-regulation or lack of Kiss-1（one of metastasis suppressor genes） and metastasis of lymph node in non-small cell lung cancer（NSCLC）.Methods The expression of Kiss-1 and Podoplanin which was used to show lymphatic vessel density（LVD） were detected in 68 paraffin sections of NSCLC by immunohistochemistry（Elivision Methods）.Results ①The difference of positive rate of Kiss-1 was significant between group 1（with metastasis of lymph node） and group 2（without metastasis of lymph node）,P0.05.②The difference of LVD was significant between group 1（15.73±0.74） and group 2（8.31±0.32）,P0.05.③Through logistic regression analysis,LVD have an impact on metastasis of lymph node（P0.05）,the relative risk（RR） was 2.065,the confidence interval of 95% was（1.202,3.547）.While it was no statistical significance of down-regulation of Kiss-1（P=0.569）,the confidence interval of 95% was（0.011,12.143）.There was no significantly synergy between LVD and expression of Kiss-1 to metastasis of lymph node（P0.05）.Conclusion There was no obvious synergies between down-regulation of Kiss-1 and lymphangiogenesis to metastasis of lymph node.