E-selectin及其配体介导食管癌细胞与血管内皮细胞早期粘附

The role of E-selectin and its ligands sLe A/X on the early adhesion of esophageal carcinoma cells with vascular endothelial cell

目的探讨E-选择素(E-selectin)及其配体sLe A和sLe X在食管癌细胞和内皮细胞早期粘附中的作用。方法应用粘附实验检测食管癌细胞和血管内皮细胞的粘附。结果静息状态下,人食管癌细胞株EC9706和奇静脉内皮细胞株ECV304粘附较少,内皮细胞经脂多糖激活后,与人食管癌细胞株粘附显著增加。激活内皮细胞组、E-选择素单抗(1∶200)、E-选择素单抗(1∶400)、E-选择素单抗(1∶800)组相对粘附率(5.2900±0.790)、(1.8818±0.8289)、(1.9436±0.3935)、(3.1730±0.4887),差异具有统计学意义(F值28.789,P值<0.001)。sLe A、sLe抗体处理组相对粘附率分别为(2.5436±0.6789)、(3.1286±0.8306),均较未处理(6.5170±0.9293)低,差异具有统计学意义(P<0.01)。结论E-selectin及其配体sLe A和sLe X介导食管癌细胞和内皮细胞早期粘附,可能与食管癌转移有关。

Objective To study the mechanism of metastasis of human esophageal carcinoma, the early adhesion of EC9706 and vascular endothelial eeU line ECV304 activated by LPS and the effects of monoelonal antibodies against E-seleetin and its ligands sLe A/X. Methods To learn the role of E-selectin and its ligands sLe A/X in EC9706 adhesion on ECV304 by cell adhesion. Results The augment of EC9706 cell adhesion on ECV304 was augmented by （5.2900 ±0. 790） fold by LPS 1 μg/ml. When ECV304 were pretreated by monoelonal antibody against E-seleetin half an hour before adding EC9706 cells, the extent of augment of EC9706 cells induction decreased to （1. 8818±0. 8289） ,（ 1. 9436±0. 3935 ） and （3. 1730±0. 4887） fold,at monoelonal antibody against E-seleetin eoneentrations of 1 ： 200、 1 ： 400 and 1 ： 800, respectively. The reduction of augment of cell adhesion was statistidy significant （P〈0. 001 ）. The augment of cell adhesion in group of stimulated ECV304, monoelonal antibody against sLe A（1 ： 100） ,monoelonal antibody against sLe X （1 ： 100） were （6. 5170±0. 9293）,（2. 5436±0. 6789） and （3. 1286±0. 8306） fold. statisfiely significant difference from group with monoelonal antibody and without monlelonal antibody（ P 〈0. 01 ）. Conclusion E-seleetin and its ligands sLe A./X play an important role in mediating the early adhesion of esophageal carcinoma and vascular endothelia cell.