摘要
利用响应面分析法(Response Surface Methodology)对羊血中SOD的热变性提纯工艺进行优化。在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用3因素水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以SOD的比活力为响应值作响应面和等高线。在分析各个因素的显著性和交互作用后,得出最佳工艺条件为:热变性温度55℃;热变性时间15min;0.01mol/lCuCl2的添加量2%。在最佳工艺条件下,SOD活性为1072.43U/mg蛋白。
Based on single factor tests, the optimum heating denatureation extraction conditions of SOD from sheep blood were obtained through Box- benhnken central combination design and RSM. The results showed that the optimum heating denatureation extraction conditions of SOD are as follows： heating denatureation temperature 55℃, heating denatureation time 15min and 2%. Under such conditions,the activity exceeds 3 000 U/mg protein was reached.
出处
《肉类研究》
2009年第11期25-30,共6页
Meat Research
基金
宁夏自然科学基金
关键词
羊血
热变性提取
响应面分析法
优化
sheep blood
heating denatureation extraction
response surface methodology
optimization
