摘要
目的了解2008年广西一起甲型肝炎暴发流行株基因型。方法采自甲型肝炎暴发流行中3所学校现症病人的血清,经RT-PCR对HAV-VP1区核苷酸进行扩增和序列分析。结果来自3所学校的HAV流行株VP1区核苷酸序列同源性为100%,与AH2株的同源性最高,为98.6%,同属于IA亚型;氨基酸水平上没有发生氨基酸的缺失、插入,未发生有意义的突变。结论IA亚型是导致此次甲肝暴发的流行株,3所学校具有相同的传染源和流行株。人群甲肝疫苗免疫水平低是甲肝流行的主要因素,应加强甲肝疫苗的免疫水平及甲肝分子流行病学监测。
Objective To investigate the genotypes of wild-type hepatitis a virus isolated in an outbreak of HAV in Guangxi,2008. Methods All serum specimens were the nucleotide sequence of VP1 region amplified by RT-PCR. Results All 3 isolated in the outbreaks belonged to genotype I and subtpye IA,the identity were as high as 100%. Conclusion All the isolated wild-type HAV strains in the outbreak were subtype IA.
出处
《中国热带医学》
CAS
2009年第12期2219-2220,共2页
China Tropical Medicine
