辣根过氧化物酶直接电化学行为的研究

A Studies of the Direct Electrochemical Behavior of HRP on the ODA-HMS Polyvinyl Alcohol Membrane Modified Glassy Carbon Electrode

制备了十八烷基胺六方介孔二氧化硅(ODA-HMS)/辣根过氧化物酶(HRP)聚乙烯醇凝胶膜化学修饰电极,考察了HRP在裸玻碳电极上和ODA-HMS聚乙烯醇凝胶膜修饰电极上的直接电化学行为,探讨了不同修饰方法包埋HRP对其直接电化学行为的影响。实验结果表明:HRP在裸玻碳电极上和滴涂法所制备ODA-HMS聚乙烯醇凝胶膜中的直接电化学行为较差,只呈现一不可逆的还原峰,且峰电流随着扫描次数的增加不断减小,无法达到稳定。而通过吸附包埋法所制备的ODA-HMS/HRP化学修饰电极上,HRP可以呈现出良好的,可逆的直接电化学行为,其氧化峰和还原峰的峰电流在数次扫描后能达到稳定。探讨了该修饰电极对双氧水的电催化还原作用。

In this paper, octadecylamine hexagonal mesoporous silica （ODA-HMS） polyvinyl alcohol （PVA） membrane modified electrode was prepared. The direct electrochemical behaviors of horse radish peroxide （HRP） on the bare glassy carbon electrode and the 0DA-HMS PVA membrane modified elec- trode were studied,respectively. The experiment results showed that the HRP could present better behavior of direct electron transfer on the ODA-HMS PVA membrane modified electode prepared by adsorption method than on the bare glassy carbon electrode, and the modified electrode prepared by dipcoating method.