摘要
制备了十八烷基胺六方介孔二氧化硅(ODA-HMS)/辣根过氧化物酶(HRP)聚乙烯醇凝胶膜化学修饰电极,考察了HRP在裸玻碳电极上和ODA-HMS聚乙烯醇凝胶膜修饰电极上的直接电化学行为,探讨了不同修饰方法包埋HRP对其直接电化学行为的影响。实验结果表明:HRP在裸玻碳电极上和滴涂法所制备ODA-HMS聚乙烯醇凝胶膜中的直接电化学行为较差,只呈现一不可逆的还原峰,且峰电流随着扫描次数的增加不断减小,无法达到稳定。而通过吸附包埋法所制备的ODA-HMS/HRP化学修饰电极上,HRP可以呈现出良好的,可逆的直接电化学行为,其氧化峰和还原峰的峰电流在数次扫描后能达到稳定。探讨了该修饰电极对双氧水的电催化还原作用。
In this paper, octadecylamine hexagonal mesoporous silica (ODA-HMS) polyvinyl alcohol (PVA) membrane modified electrode was prepared. The direct electrochemical behaviors of horse radish peroxide (HRP) on the bare glassy carbon electrode and the 0DA-HMS PVA membrane modified elec- trode were studied,respectively. The experiment results showed that the HRP could present better behavior of direct electron transfer on the ODA-HMS PVA membrane modified electode prepared by adsorption method than on the bare glassy carbon electrode, and the modified electrode prepared by dipcoating method.
出处
《上海应用技术学院学报(自然科学版)》
2009年第3期223-228,共6页
Journal of Shanghai Institute of Technology: Natural Science
基金
上海市自然科学基金项目(07ZR14106)
上海高校选拔培养优秀青年教师科研专项基金资助基目(yyy-07004)
上海应用技术学院引进人才科研启动资助项目(YJ2007-20)
关键词
辣根过氧化物酶
化学修饰电极
介孔材料
电化学
horseradish peroxidase
mesoporous silica material
chemically modified electrode
electrochemistry