摘要
利用PCR技术分别扩增合浦珠母贝(Pinctada fucata)、长耳珠母贝(P.chemnitzi)和企鹅珍珠贝(Pteria penguin)的16S rRNA基因片段,PCR产物直接测序,删去引物及部分端部序列后,得到439bp可供分析的核苷酸片段,用MEGA3.1软件分析了核苷酸差异。结果表明,合浦珠母贝种内个体间序列完全相同,长耳珠母贝种内个体间有2个颠换(Transversion)突变位点,而企鹅珍珠贝种内个体间有1个转换(Transision)突变位点,5个颠换(Transversion)突变位点。与GenBank数据库中其他9种珍珠贝的序列进行比较,得到464个同源比对位点,包括51个插入/缺失位点和231个变异位点(173个简约信息位点和53个单突变子)。合浦珠母贝与长耳珠母贝的同源性为83.2%,合浦珠母贝、长耳珠母贝与企鹅珍珠贝的同源性分别为55.4%和59%。NJ系统进化树表明合浦珠母贝和长耳珠母贝与珠母贝属的种类聚在一起,而企鹅珍珠贝与珍珠贝属的种类聚在一起,与形态学分类一致。
Mitochondrial 16S rRNA gene fragments were amplified from Pinctada fucata, P. chemnitzi and Pteria penguin using PCR technique. The PCR products were directly sequenced, and 439 bp nucleotide sequences were obtained after excluding the primers and truncating partial sequences at the end. Sequence variation was analyzed using MEGA 3.1 software. The results indicated that base substitution was not found among P. fucata, but two transversions were found among P. chemnitzi, one transition and five transversions among P. penguin. Alignment with nine homologous sequences from Pteridae species in GenBank revealed 51 insertion/deletions and 231 variable sites ( 173 parsimony-informative sites and 53 singletons) among 464 truncated alignment sites. The percentage of the identity of the 16S rRNA gene fragments was 83.2% between P. fucata and P. chemnitzi, 55.4% between P. fucata and Pteria penguin, and 59% between P. chemnitzi and P. penguin. The NJ tree indicated that P.fucata and P. chemnitzi were assembled together with Pinctada species, yet P. penguin assembled together with the Pteria species, which was consistent with the morphological classification.
出处
《南方水产》
2009年第6期47-53,共7页
South China Fisheries Science
基金
国家科技支撑计划项目(2006BAD01A13)
国家高技术研究发展计划(863计划)项目(2006AA10A409)
广东省科技计划项目(2006B20201063)