广西各地岩黄连药材中脱氢卡维丁含量考察

Study of Dehydrocavidine in Herbs of Corydalis Saxicola Bunting from Ten Counties of Guangxi

目的考察广西10个县产岩黄连中脱氢卡维丁的含量,为控制岩黄连药材的质量提供参考。方法将岩黄连药材全草粉碎,加入无水乙醇,经索氏提取至无色,取药液过滤、稀释,采用高效液相色谱(HPLC)法测定脱氢卡维丁含量。色谱柱为Platisil-ODS柱(250mm×4.6mm,5μm),流动相为乙腈-0.02mol/L磷酸二氢钾(80∶20,V/V),流速为0.5mL/min,检测波长347nm。结果脱氢卡维丁质量浓度在2.50～20.96μg/mL范围内与峰面积线性关系良好(r=0.99995),最低检测质量浓度为0.25μg/mL(以S/N>4计)。平均加样回收率为98.63%,RSD=0.89%(n=6)。广西各县产的岩黄连药材含脱氢卡维丁有差异,其中乐业、靖西、东兰、都安、环江等县的药材含量较高。结论HPLC法测定脱氢卡维丁含量简便可行,可用于控制岩黄连药材质量。

Objective To investigate the dehydrocavidine content in the medicinal herb, Corydalis saxicola Bunting, produced in ten counties of Guangxi, China, Methods To crush the herbs of Corydalis saxicola Bunting, add anhydrous ethanol into it and extract it until it is colorless by Soxhlet extraction, then filtrate and dilute the herbs liquid. HPLC was used to determine the content of dehydrocavidine. It was performed on the Platisil - ODS column （250 mm ×4.6 mm, 5 μm）, CH3CN -0. 02 mol/L KH2PO4（pH =3） （80 ： 20, V/V） served as the mobile phase and the detection wavelength was at 347 nm, the column temperature at 25℃ and the flow rate 0. 5 mL/min. Results The regression equation showing linear relationship between peak-area and concentration of dehydrocavidine in the range of 2.50-20.96μg/mL（ r= 0. 999 95）. The detection limit was 0.25 μg/mL（ S/N 〉 4）. The recovery rate was 98.63%, RSD 0. 89% （ n = 6）. The contents of dehydrocavidine in herbs of Corydalis saxieola Bunting from ten counties of Guangxi were different, which in five counties as Leye, Jingxi, Donglan, Du＇ an, Huanjiang were higher than those in other counties. Conclusion This method has good sensitivity, precision and simplicity. It is shown to be suitable for the quality control of Corydalis saxicola Bunting.