液相色谱-质谱联用法检测壮阳制剂中非法掺入的伪伐地那非

Detection of Pseudo-Vardenafil Illegally Added into Anti-Impotence Preparations by LC-MS

目的建立检测壮阳制剂中非法掺入的伪伐地那非的专属性方法。方法将壮阳制剂的提取液通过液相色谱-串联四极杆质谱分析,根据相对分子质量、二级质谱碎片信息、液相色谱保留时间、紫外光谱等信息,对壮阳制剂中非法掺入的伪伐地那非进行定性鉴别。结果检测到壮阳制剂中掺有伪伐地那非。结论液相色谱-质谱联用法专属性强,灵敏度高,可作为检测壮阳制剂中非法掺入伪伐地那非的有效方法。

Objective To develop a specific method for the identification of pseudo- vardenafil illegally added into antiimpotence preparations. Methods The liquid chromatography （LC）- quadrupole mass spectrometry （MS） method was used. After separated by HPLC, the organic extraction of the sample was analyzed. The compounds illegally added into antiimpotence preparations were identified according to the spectrum, chromatographic behavior and mass spectral data by comparison with those of reference substances. Results Pseudo- vardenafil was found in anti- impotence preparations. Conclusion The method is selective and sensitive, which can be used to detect pseudo- vardenafil illegally added into anti- impotence drugs and health products.