利用Affymetrix芯片分析DON诱导抗赤霉病小麦品种望水白的基因表达谱

Gene Expression Analysis Upon DON Infection on a Scab Resistant Wheat Variety Wangshuibai by Affymetrix Wheat Chip

中国小麦地方品种望水白不但高抗赤霉病,而且其籽粒中DON(脱氧雪腐镰刀菌烯醇,被认为是赤霉病的致病因子)含量也低。为了研究望水白低DON含量的分子机制,利用Affymetrix小麦基因芯片对望水白受DON诱导调控的基因进行高通量的检测,以DON诱导后12 h和24 h混合样品作为处理组,水处理做为对照。总共检测到差异表达的基因1 114个,其中上调表达的基因有949个,下调基因165个。在上调表达基因中,推断有功能的基因涉及转录因子、信号蛋白、着丝粒蛋白以及与病程相关的基因,如:腺苷三磷酸结合盒转运蛋白,谷胱甘酞转移酶、细胞色素P450酶、苯丙氨酸解氨酶、葡萄糖基转移酶以及抗病蛋白等。对上调表达中的部分基因进行RT-PCR分析,证实它们都受DON诱导上调表达,与芯片检测结果吻合。

Fusarium heaFusarium grarninearum reduce yield and its production of the trichothecenes toxins such as deoxynivalenol （DON） is very harmful to both human and animal health. Wangshuibai,a local variety of Jiangsu Province, shows both high level of scab resistance and low level of DON content. In order to understand the molecular mechanism of low DON content of Wangshuibai, gene profiling was performed using Affymetrix Wheat Chip on equally mixed RNA samples from 2 time points （12 h, 24 h after DON or water treatment, respectively） of spikes. A total of 1 114 genes were found to be differentially expressed depending on the comparison when the ＂signal ratio value＂ was set as more than 2. Among them, 949 genes were upregulated and 165 were downregulated. Annotation results found that 496 upregulated genes have function description, including transcription factor, signal protein, centromere protein and many patho genesis-related genes such as ATP-Binding Cassette transporter （ABC transporter）, glatocnine S-tranferases （GSTs）, Cytochrome p450 （p450）, phenylalanine ammonialyases （PAL）, UDP-glucosyltransferases （UGTs） and resistance proteins. Several up regulated genes were selected for RT PCR analysis, and the results showed that their expression patterns were consistent with the result of Gene chip hybridization. The information will be helpful for the cloning of DON resistance related genes, and characterize molecular mechanism of DON resistance.