新疆11种豆科作物立枯丝核菌菌丝融合群及营养亲和群研究

Anastomosis groups and vegetative compatible groups of Rhizoctonia solani from 11 leguminaceous crops in Xinjiang

从新疆11种豆科作物病株上或病株根围土样中分离纯化出250个立枯丝核菌(Rhizoctonia DC),番红O-KOH染色后观察细胞核数目,经测试全部菌株均为多核,用标准菌株测定融合群,250个菌株分属为AG-1、AG-2、AG-3、AG-4和AG-5共5个融合群,出现频率分别为16.4%、33.2%、0.4%、32.4%和17.6%,营养亲合群判别结果表明,AG-1、AG-2、AG-4和AG-5下各有2个VCG,说明新疆豆科作物立枯丝核菌各主要菌丝融合群内均有不同程度的分化。

Two hundreds and fifty fungal strains isolated from diseased tissues of 11 leguminaceous crops and soils in Xinjiang were identified as Rhizoctonia solani. By using safranin O-KOH staining method, all of the strains were determined to belong to multinucleate R. solani. According to the results of standard anastomosis groups test, 250 of the isolates could be divided into 5 anastomosis groups as follows： AG-1, AG-2, AG-3, AG-4 and AG-5, with frequencies of 16.4%, 33.2%, 0.4%, 32.4% and 17. 6%, respectively. AG1, AG2, AG4 and AG5 could be divided into 2 vegetative compatibility groups. The results showed that the anastomosis groups of R. solani isolated from leguminaceous crops in Xinjiang had intra-species differentiation to some extent.