不同铜源对大鼠生长性能及组织中铜含量和血清中抗氧化酶活性的影响

Effect of Different Copper Resources on Growth Performance and Copper Content in Tissues and Antioxidant Enzymes in Serum for Rats

比较酵母铜作为新型铜源添加剂与硫酸铜在大鼠上的饲喂效果。选择血缘相近,体重在60～90g的Wistar健康雌鼠66只,按2因子完全随机设计,分为11个处理,每个处理6个重复,试验期10d。采用酪蛋白、玉米淀粉纯合缺铜饲粮作为基础饲粮,分别以酵母铜和硫酸铜2种不同的铜源添加10、30、60、100、250mg/kg5个水平。结果表明:不同铜源、不同铜水平、不同铜源与不同铜水平互作之间,大鼠的平均日增重(ADG)、平均日采食量(ADFI)差异均不显著(P>0.05);但是酵母铜与硫酸铜相比,ADG和ADFI分别提高了2.5%和3.5%。不同铜源、不同铜水平、不同铜源与不同铜水平交互作用,对大鼠血清铜蓝蛋白、铜锌超氧化物歧化酶(CuZn-SOD)、肝铜含量都存在极显著的影响(P<0.01)。由此可见,不同铜源和水平能够显著影响大鼠对铜的吸收利用,且酵母铜源较硫酸铜源的效果更优。

Sixty-six Wistar female rats weighted 60-90 g were allotted to 11 treats with 6 replications, 1 rats per replicate in a completely randomized design according to two factorial experiment arrangement. 10, 30, 60,100,250 mg/ kg copper either from copper-enriched yeast or copper sulfate were added to the purified basal diet. The trial duration was 10 days after 12-day deletion. The results indicated that there were no significant difference between copper resources and treat level and copper resources x treat level in ADG or ADFI, but ADG and ADFI were increased by 2.5% and 3.5% respectively for copper-yeast treatments and copper sulfate treatments. There were extremely significance on ceruloplasmin, CuZn-SOD, liver-copper between copper resource among copper levels and copper resource x copper level. The results indicated that copper from yeast had higher bioavailability than that from copper sulfate.