花生过敏原Ara h2.02二级结构和B细胞抗原表位预测

Prediction of Secondary Structure and B Cell Epitopes of Peanut Allergen Ara h 2.02

为预测花生过敏原Arah2.02的二级结构和B细胞抗原表位,通过Genbank数据库搜索到Arah2.02基因,并推导出其相应的氨基酸序列,采用SOPMA和DNAStar软件预测该蛋白质的二级结构以及通过Jameson-Wolf法、Kyte-Doolittle法、Emini法和Karplus-Schulz法分别预测其抗原指数、亲水性、表面可及性、柔韧性等参数,并综合分析预测该蛋白的B细胞抗原表位。结果表明:在序列28～31、56～73、76～90、92、148、156～158、168～169区域最可能是Arah2.02蛋白的B细胞抗原表位的优势区域。该结果将为寻找Arah2.02的最佳优势表位提供支持,同时为该蛋白单克隆抗体的制备等研究提供理论依据。

In order to predict the secondary structure and B cell epitopes of peanut allergen Ara h 2.02, the gene of Ara h 2.02 was searched in Genbank and its amino sequence was deduced. Meanwhile, its secondary structure was predicted with DNAStar and SOMPA, and antigenicity. hydrophilicity, surface probability and flexibility was predicted using the methods of Jameson- Wolf, Kyte-Doolittle, Emini and Karplus-eSchulz, respectively. B-cell epitopes of Ara h 2.02 were further predicted based on the comprehensive prediction analysis. Results indicated that B-cell epitopes had the high probability in the regions of 28-31,56 - 73, 76 - 90, 92, 148, 156 - 158, and 168 - 169. These investigations will be beneficial to epitope localization ofAra h 2.02 and monoclonal antibody preparation in the future.