紫外线与亚硝基胍复合诱变选育高产海藻糖菌株的研究

UV/NTG Mutagenesis for Breeding of A High Trehalose-producing Strain of Saccharomyces cerevisiae

本实验以酿酒酵母为出发菌株,用紫外线与亚硝基胍复合诱变选育出一株遗传稳定性较好的高产海藻糖菌株,其海藻糖含量为19.8%(以干酵母计),比原菌株提高了39.43%,比只用紫外线诱变后的菌株提高了10.0%,比只用亚硝基胍诱变后的菌株提高了6.45%。

A high trehalose-producing strain of Saccharomyces cerevisiae was bred via combined nitrosoguanidine （NTG）/UV mutagenesis. This strain had good genetic stability and a trehalose content of 19.8% （m/m dry yeast）, which revealed the enhancement of 39.43%, 10.0% and 6.45% compared with the original strain, the strain screened by alone UV mutagenesis, and the strain screened by alone NTG mutagenesis, respectively.