摘要
为得到高效特异的抗卵转铁蛋白多克隆抗体,通过用商品化的卵转铁蛋白(OVT)标品和自制纯化的OVT免疫新西兰大白兔,用ELISA及Western blotting方法检测制备的抗血清效价和特异性。结果表明获得的抗体效价均高达1:819200以上,抗体特异性强。本实验所建立的方法可成功制备高效特异的抗卵转铁蛋白多克隆抗体,可为进一步研究提供实验材料。
In order to prepare highly specific rabbit anti-ovotransferrin polyclonal antibody, New Zealand rabbits were immunized with commercial ovotransferrin or laboratory-purified ovotransferrin. The titer and specificity of antisera prepared were analyzed by ELISA and Western blotting, respectively. Results showed that all the antisera reached a titer of 1：819200 and exhibited a high specificity. Therefore, target products have been successfully prepared, which might be useful for the further investigations of egg allergy.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第21期214-216,共3页
Food Science
基金
江西省自然科学基金项目(2008GZN0040)
南昌大学食品科学与技术国家重点实验室自由探索项目(SKLF-TS-200820)
江西省研究生创新专项资金项目
关键词
卵转铁蛋白
多克隆抗体
鸡蛋过敏
ovotransferrin （OVT）
polyclonal antibody
egg allergy
