摘要
研究制备能有效用于米诺环素(MNC)残留检测的完全抗原。采用戊二醛合成法和重氮化合成法,将米诺环素与牛血清白蛋白(BSA)偶联,分别制备MNC完全抗原MNC-BSA。经红外和紫外光谱扫描结果表明:两种物质已经偶联,两种完全抗原的平均偶联比分别为9.9和5.9。采用戊二醛法合成的完全抗原免疫兔,检测抗血清效价为1:16000,表明合成的完全抗原可以作为免疫原用于制备抗体及检测时的包被原进行ELISA检测。
In order to effectively detect minocycline （MNC） residues, a complete antigen of MNC was developed by glutaraldehyde synthesis and diazotization methods. MNC was coupled with bovine serum albumin （BSA） to obtain a complete antigen of MNC-BSA. Infrared and ultraviolet scanning spectral analysis confirmed that the complete antigen of MNC-BSA had been successfully coupled by both methods, with average conjugation ratios of 9.9 and 5.9, respectively. The serum titer of rabbit immunized with MNC-BSA competent antigen prepared by glutamldehyde synthesis method was 16000, which indicated that the synthesized complete antigen could not only be used as the immunogen to prepare the corresponding antibody, but also act as the coating antigen for enzyme linked immunosorbent assay （ELISA） kit.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第21期305-308,共4页
Food Science
基金
辽宁省教育厅重点项目(2009A633)
关键词
米诺环素
牛血清白蛋白
偶联
完全抗原
minocycline
bovine serum albumin （BSA）
conjugation
complete antigen
