杜仲雄花乙酸乙酯提取物镇静催眠作用研究

Sedative and Hypnotic Effect of Ethyl Acetate Extract from Male Flowers of Eucommia ulmoides Oliv

目的:研究不同剂量杜仲雄花乙酸乙酯提取物的镇静催眠作用,确定杜仲雄花镇静催眠活性部位并分析其有效作用剂量。方法:观察不同剂量乙酸乙酯提取物对小鼠自主活动的影响以及直接镇静催眠作用、与戊巴比妥钠的协同作用和抗惊厥作用。结果:杜仲雄花乙酸乙酯提取物能显著增加小鼠睡眠率,减少小鼠的自主活动次数;显著增加阈下剂量戊巴比妥钠小鼠睡眠率,延长阈上剂量戊巴比妥钠睡眠时间,缩短入睡潜伏期;减少惊厥小鼠数,延长惊厥潜伏期。结论:杜仲雄花乙酸乙酯提取物具有良好的镇静催眠作用,是杜仲雄花镇静催眠活性的有效组分。

The aim of this study was to investigate the sedative and hypnotic effect of ethyl acetate extract from male flowers of Eucommia ulmoides Oliv and to determine its optimal effective dose using Kunming male mice as experimental animals. The effect of the extract on spontaneous activity in mice, possible synergetic effect with pentobarbital sodium and anticonvulsant effect of with different doses were analyzed. Results showed that the ethyl acetate extract was able to significantly induce sleep in mice, inhibit the spontaneous activity, enhance the percentage of sleeping mice with the treatment of subthreshold dose of pentobarbital sodium, prolong the sleeping time induced by superthreshold dose of pentobarbital sodium and the convulsion latency, shorten the sleep latency, and reduce the eclamptia ratio in mice. In summary, ethyl acetate extract from male flowers ofEucommia ulmoides Oliv possess obviously sedative and hypnotic effects in mice.