利用GiemsaC-带和45SrDNAFISH的方法鉴定百合杂种

Identification of Lilium hybrid by Giemsa C-banding and 45S rDNA FISH

利用Giemsa C-带和染色体荧光原位杂交(45S rDNA FISH)的方法对‘Royal Lace’בHigh Class’的杂种后代分别进行了鉴定。结果表明:‘Royal Lace’的染色体数2n=3x=36,‘High Class’的染色体数2n=2x=24。杂种后代的染色体数出现2n=3x=36和2n=4x=48两种类型。经Giemsa C-带和45S rDNA FISH方法对两个亲本和具2n=4x=48的杂种后代分析结果表明,杂种后代的根尖染色体C-带可观察到来自双亲的可以特异追踪的染色体带纹。FISH分析表明,杂种后代中分别有两条来自‘Royal Lace’和‘High Class’的染色体。两个亲本的荧光原位杂交点数分别为10和9。杂种后代染色体为2n=36的个体有14个杂交信号,可以确定两条来自‘Royal Lace’,另外3条来自‘High Class’。杂种后代中2n=48的个体的杂交信号点为19,从而证实所获得的杂种后代的真实性。同时,对于2n=48,而杂交信号为19的多倍体起源于未减数分裂的2n雄配子体的可能性进行了探讨。综合研究结果表明,Giemsa C-带和45S rDNA FISH方法可以准确地对百合的杂种真实性进行鉴定。

In this study, hybrid plants from the crosses hetween ＇ Royal Lace＇ x ＇ High Class＇ were analyzed with Gi- emsa C - banding and 45S rDNA FISH. The results showed that, the number of chromosomes was 2n = 3x = 36 for ＇Royal Lace＇ , 2n=2x=24 for ＇High Class＇ and2n=3x=36 or2n=4x--48 for two different hybrids, respectively. The special C -banging patterns of two parents can be found in their hybrids. By FISH analysis, 14 hybridization locies were detected in the hybrids of 2n = 36. Two chromosomes from ＇ Royal I.ace＇ and three chromosomes from ＇ High Class＇ can be identified clearly. And there were 19 hybridization locies to be distinguished for 2n = 48 hybrids,which also suggested that these hybrids might relate to be the contributed by unreduced male gametes （2n）. All of these evidences clearly confirmed that, the plants were true hybrids.