铜绿假单胞菌的临床感染及耐药性分析

Investigation on the infective status and the drug resistance of Pseudomonas aeruginosa

目的了解铜绿假单胞菌(PAE)临床感染特点和耐药特性,为医院感染的监测与控制提供依据。方法采用法国生物梅里埃公司的API鉴定系统及VITEK2系统进行细菌鉴定,用K-B纸片扩散法进行药敏试验,用WHONET 5.4软件分析PAE的耐药性。结果10年来共分离出2479株PAE,主要来源于病房的呼吸道标本,其对23种抗菌药物的耐药率逐年上升,只有美罗培南、亚胺培南、头孢他啶和环丙沙星的耐药率<30%,多重耐药PAE共有188株,主要分布在重症监护病房、呼吸内科。结论PAE的耐药性已十分严重,必须进行严密监控,以预防PAE导致的医院感染暴发流行。

Objective To investigate the clinical characteristics of Pseudomonas aeruginosa（PAE） infection and its drug resistance to provide the evidence for clinical monitoring and control of nosocomial infection.Method PAE was identified by API and VITEK2 system;its antimicrobial ressistance was determined by Kirby-Bauer method.The antimicrobial ressistance rates were analyzed by WHONET 5.4 software.Result A total of 2479 strains of PAE were isolated and collected in ten years,which mostly existed in samples from respiratory tract. The ressistance of PAE to 23 antibiotics had increased in the past ten years. The resistant rates to meropemem,imipenem, eiproftoxaein and ceftazidime were below 30%. Among the 2479 strains of PAE, 188 strains were identified multi-drug resistant and found more frequently in ICUs and Departments of Respiration. Conclusion The drug resistance of PAE is very serious in our hospital. We should perform rigorous monitoring and prevent the outbreak of nosocomial infection by PAE.