抗表皮生长因子受体单克隆抗体联合5-氟尿嘧啶、健择对胰腺癌的作用

The effect of anti-EGFR monoclonal antibody ,5-Fu and gemcitabine therapy on the growth of hu- man pancreatic cancer cells in nude mice model

目的观察抗表皮生长因子受体（EGFR）单克隆抗体联合5-氟尿嘧啶（5-Fu）、健择对胰腺癌的作用。方法将人胰腺癌肿瘤细胞悬液注射于裸鼠背部皮下，建立肿瘤模型。干预组腹腔注射鼠抗人EGFR单克隆抗体（MMAb-2）、5-Fu、健择及其配伍联合用药；对照组注射生理盐水。干预4周后处死裸鼠，评价干预效果。结果MMAb-2单独应用对胰腺癌细胞增殖有抑制作用（P〈0．05），MMAb-2联合应用5-Fu、健择对胰腺癌细胞增殖的抑制作用明显增强（P〈0．05），MMAb-2组裸鼠体质量及其白细胞计数明显高于化疗组（P〈0．05）。结论抗EGFR抗体单独应用有确切的抗肿瘤疗效；与化疗联合应用可以增效；对裸鼠机体免疫功能无明显毒性。

Objective To study the effect of anti-EGFR monoclonal antibody （MMAb-2） therapy on the growth of pancreatic cancer cells in combination with chemotherapeutic agent. Methods A tumor cell suspension （SW1990） was injected into the subcutaneous dorsa of mice in the proximal midline. The treatment groups received intraperitoneal injections of MMAb-2 or 5-Fu or gemcitabine or MMAb-2,5-Fu and gemcitabine in combination respectively. The control group only received intraperitoneal injection of saline. After 4 weeks for treatment, the mice were killed. Results The inhibition ratio of proliferation of pancreatic tumor cells was increased evidently by 5-Fu, gemcitabine in combination with MMAb-2 （P 〈 0.05）. Conclusion The MMAb-2 could enhance significantly the sensitivity of the cyto-inhibition of the chemotherapeutic agents on pancreatic tumor cells, and improve the chemotherapeutic effects of pancreatic cancer cells.