不同浓度TH黏蛋白对草酸钙成核、生长、聚集的影响

The effect of Tamm-Horsfall Mucoprotein on the nucleation, growth and aggregation of calcium oxalate monohydrate crystals in vitro

目的观察不同浓度TH黏蛋白（THM）对体外成石系统内-水草酸钙（COM）的成核、生长和聚集的影响。方法盐析法提取THM，利用原子分光光度计测定0、10、50、100mg／LTHM人工尿体外成核和种籽晶溶液内Ca2＋变化，偏光显微镜计数COM数目。结果THM为0、10、50、100mg／L时，Ca2＋减少量0．81、0．57、0．51、0．96mmol／L；成核数3．20×10^4、2．32×10^4、1．83×10^4、2．85×10^4；生长率53．9％、48．7％、34．6％、19．8％；聚集率33．2％、11．7％、9．4％、66．2％，差异均有统计学意义（P〈0．01）。结论在生理浓度范围内，THM可以抑制尿结石的形成，且随浓度增加其抑制作用逐渐增强，但超过一定浓度时，其转为结石促进剂。

Objective To determine the effect of Tamm-Horsfall Mucoprotein （THM） on the nucleation,growth and aggregation of calcium oxalate monohydrate crystals （COM） in vitro. Methods THM was isolated from normal individuals according to the method of Tamm and Horsfall with a little modification. The concentration of calcium and the amount of COM crystals were determined in the artificial urine and seed crystal system with 0, 10,50,100 mg/L THM. Results Under the concentrations of THM being 0,10 ,50 ,100 mg/L, the reductions of the concentration of calcium were 0. 81,0. 57,0. 51,0. 96 mmol/L; the amounts of nucleation were 3.20 × 10^4, 2.32 × 10^4, 1.83×10^4, 2.85 ×10^4 ; the growth rate was 53.9% ,48.7% ,34.6% , 19.8% ; the aggregation rate was 33.2% , 11.7%, 9.4%, and 66.2% respectively. There was significant differences, P 〈 0.01. Conclusion THM could prevent the calcium oxalate crystals from nucleating, growing and aggregating with the physiological concentration range, but when the concentration of THM got a top limit of the physiological concentration, THM became a promoter of nucleating and aggregating of calcium oxalate crystals.