人乳腺癌组织中血小板活化因子的表达及临床意义

Expression and significance of platelet-activating factor in human breast cancer

目的观察人乳腺癌组织血小板活化因子（PAF）的表达。方法应用免疫组织化学染色检测PAF在10例晚期乳腺癌及其癌旁正常组织中的分布和逆转录一聚合酶链反应（RT—PCR）分析PAF受体mRNA表达水平。结果90％（9／10）的乳腺癌组织中PAF受体mRNA和PAF表达增强，而癌旁组织中80％（8／10）PAF受体mRNA和PAF表达减少，两者差异有统计学意义（P〈0．05）。结论中晚期乳腺癌中PAF表达增强，可能在乳腺癌的发病过程中发挥作用。

Objective To investigate mammalian target of platelet-activating factor（PAF） gene expressions in human breast cancer. Methods The samples were obtained from 10 cases with human breast cancer specimens. Immunohistochemical analysis and Semi-quantitative RT-PCR strategies were used for analyzing the PAF positive-staining and PAF acceptor mRNA expression in 10 samples and adjacent noncanerous tissues. Results PAF positive-staining and PAF acceptor mRNA were detected in 90 % of human breast cancer and in of 20% adjacent noncanerous tissues （P 〈 0.05）. Conclusion PAF elevation might be in human breast cancer. PAF may play a role in the pathogenesis of haman breast cancer.