大鼠肝损伤动物模型的建立

Establishment of the surgical hepatic lesions model in rats

目的探讨制作SD大鼠肝损伤动物模型的方法。方法选用SD大鼠40只，随机分成对照组和模型组，每组20只，模型组切除大鼠肝脏的70％，对照组在同样的条件下打开关闭腹腔，分别在术前和术后12h、1、3、5、7、14d检测两组大鼠的肝功能指标，术后14d光镜观察肝脏的病理变化。结果模型组肝功能指标AST、ALP和DBIL在术后12h至7d，AIJT和TB在术后12h至5d与对照组比较差异有统计学意义（P〈0．05），至术后14d两组大鼠肝功能各项指标比较差异均无统计学意义（P〉0．05）；术后14d光镜观察大鼠肝脏组织切片，对照组大鼠肝细胞之间排列疏松，细胞棱角较锐利，肝窦较宽，模型组大鼠出现肝细胞水肿，肥大，嗜酸性变的代偿性病理变化。结论切除大鼠肝脏总体积的70％可有效建立大鼠肝损伤的模型。

Objective To explore the methods of establishing the model of surgical hepatic lesions in rats. Methods 40 SD rats,distributed into 2 groups randomly,The model group （n =20） ：to be resected 70% of the whole liver;the control group （n =20） ：to be opened and closed the peritoneal cavity under the same condition, the levels of ALT, AST, ALP, TB, DBIL of the both groups were assayed pre-operation and at 12 hours and days 1,3,5,7,14 after operation respectively. Results The levels of AST, ALP, DBIL were statistical significanees in the model group within 12 h to 7 d and ALT,TB were statistical significanees within 12 h to 5 d after operation compared with the control group （P 〈 0.05 ）. There were no statistical significances for all of the indicators between both groups at the 14th d. The histological slices of the liver section of the control group showed that it was loose among the hepatocytes, the edge of which were sharp, the hepatic sinusoid was wider, whereas the model group indicated that the hepatocytes ,were hydropic,hypertrophic,eosinophilic change. Conclusion To resect 70% volume of the whole liver of SD rats, which could establish the model of the surgical hepatic lesions effectively.