卵巢癌组织中Bmi-1蛋白的表达与端粒酶活性的关系

Correlation of Bmi-1 expression and telomerase activity in ovarian cancer

目的通过检测癌基因Bmi-1在卵巢上皮癌中表达情况，探讨其与端粒酶活性的关系。方法①使用Reahime—PCR的方法检测47例卵巢上皮癌组织中Bmi-1基因的mRNA表达情况；②采用免疫组化SP法检测47例卵巢上皮癌组织中Bmi-1蛋白的表达；③改良端粒重复序列扩增技术（telomerase repeat amplification protocol）——TRAP-银染技术检测该47例卵巢上皮癌组织中端粒酶基因水平；④Western Blot检测卵巢上皮癌组织中端粒酶蛋白表达量。结果①Bmi-1蛋白在卵巢上皮癌组织中较正常组织有明显表达（P〈0．05），并且与病理分级和临床分期有关，G3级阳性表达率（93．10％）高于G2级（61．11％），Ⅱ期、Ⅲ期阳性表达率（66．67％）低于Ⅳ期（92．31％），二者均有统计学差异（P〈0．05）。②卵巢上皮癌组织中端粒酶活性阳性检测率为87．23％（41／47），正常组织中阴性表达；③端粒酶活性阳性标本中Bmi-1蛋白高表达，占90．24％，spearman相关性分析显示Bmi-1蛋白表达与端粒酶活性阳性率呈正相关（P〈0．05）。结论Bmi-1基因和蛋白在卵巢上皮癌组织中高表达，其表达率与组织学分级和临床期别相关；Bmi-1阳性率与端粒酶活性阳性率密切相关，其在卵巢癌的发病过程中可能有重要意义．值得进一步研究。

Objective To detect the correlation between the expression of oncogene Bmi-1 gene and telomerase activity in ovarian cancer. Methods RT-PCR method and SP immunohistochemistry were adopted to detect the mRNA of Bmi-1 gene and the expression of Bmi-1 protein in tissues of 47 ovarian epithelial cancer cases. TRAP-silver staining technique was used to detect the telomerase activity. Western Blot was adopted to detect the expression of Telomerase hTERT. Results Compared with the normal ovary epithelium tissues, Bmi-1 protein in tissues of 47 ovarian epithelial cancer cases processed more obvious expression and was related to pathological grade and clinical stage. Significant Bmi-1 expression levels were found among different clinopathological types of the cancer （ P 〈 0.05 ）. In ovarian epithelial cancer tissues, 87.23% demonstrated positive telomerase activity in contrast to zero activity in normal tissues. Majority （90.24%） of specimens with positive telomerase activity possessed high Bmi-1 expression levels. Spearman correlation analysis indicated that expression of Bmi- 1 protein was positively correlated with the elevated telomerase activity. Conclusion Bmi-1 protein is highly expressed in ovarian epithelial cancer tissues, and its expression level correlates with histological grade and clinical phase of the patients. Elevation of Bmi-1 expression is closely correlated to the increased telomerase activity, and plays a significant role in the pathogenesis of Ovarian Cancer, which deserves further researches.