P38MAPK、Caspase-8在Fas—AD诱导Bel-7402细胞凋亡中的作用

The roles of P38MAPK and Caspase-8 in apoptosis of Bel-7402 cells induced by Fas-AD

目的通过P38MAPK抑制剂SB203580及caspase-8抑制剂Ac-IEFD-cho的作用，确定P38MAPK、caspase-8的相互作用关系，进一步揭示Fas—AD诱导Bel-7402细胞凋亡的信号转导机制。方法通过RT—PCR法检测培养的Bel-7402细胞中P38MAPK mRNA、caspase．8 mRNA在Fas．AD、SB203580及Ac—IEFD—cho作用下的表达情况。结果在Fas-AD诱导Bel-7402细胞凋亡中，SB203580和Ac—IEFD—cho能分别抑制p38MAPK mRNA、caspase-8 mRNA的表达。结论在Bel-7402细胞凋亡中，P38MAPK与easpase-8参与Fas—AD凋亡途径，并且在mRNA水平进行相互调节。

Objective To confirm the involvement of P38MAPK and easpase-8 in apoptosis and to reveal the signal transducation mechanism of apoptotic Bel-7402 ceils. Methods Bel-7402 cells were treated with Fas- AD for 24h in the presence/absence of easpase-8 inhibitor Ac-IEFD-cho or SB203580. The expression of P38MAPK mRNA and caspase-8 mRNA was detected by RT-PCR. Results After Bel-7402 ceils were treated with Fas-AD in the presence of the caspase-8 inhibitor Ac-IEFD-cho or P38MAPK inhibitor SB203580, the expression of caspase-8 mRNA and P38MAPK mRNA significantly decreased. Conclusion P38MAPK and caspase-8 were involved in Fas-AD apoptotic pathway and regulated each other in mRNA level.