葛根素对缺氧缺血性脑病新生大鼠脑细胞凋亡及Bim蛋白表达的影响

Influence of puerarin on brain cell apoptosis and expression of Bim protein in hypoxic ischemic encephalopathy neonatal Rats

目的观察葛根素对缺氧缺血性脑病(HIE)新生大鼠脑细胞凋亡及Bim蛋白表达的影响。方法7日龄Wistar大鼠随机分成假手术组、HIE模型组、葛根素治疗组,每组22只,参照Rice法制作HIE模型,应用Western-blot和流式细胞术在不同时间点分别检测大脑皮质Bim蛋白的表达和细胞凋亡情况。结果HIE模型组和葛根素治疗组脑细胞Bim蛋白表达灰度值在12 h分别为137.00±5.00和78.20±4.38,24 h分别为164.40±5.50和90.40±3.36,均显著高于假手术组(29.60±3.20和30.00±3.74),葛根素治疗组Bim蛋白表达低于HIE模型组。HIE模型组和葛根素治疗组脑皮质早期凋亡细胞比例在12 h分别为(16.2±1.86)%和(8.54±1.91)%,24 h分别为(20.03±2.02)%和(13.36±1.64)%,均显著高于假手术组((0.76±0.09)%和(1.34±0.24)%),葛根素治疗组细胞凋亡低于HIE模型组。结论葛根素能抑制HIE新生大鼠脑细胞凋亡启动因子Bim蛋白表达,具有抗脑细胞凋亡作用。

Objective It is to observe the influence of puerarin on brain cell apoptosis and the expression of Bim protein in hypoxie ischemic encephalopathy （HIE） neonatal Rats. Methods Seven-day neonatal Wistar Rats were randomly divided into sham operated group, HIE model group and puerarin treatment group in which there were 22 Rats. HIE model was made in accordance with Rice method. The expression of Bim protein and apoptosis in cerebral cortex was respectively detected with western-blot and flow cytometry in different time point. Results The grayscale value of the expression of Bim protein in brain cell in HIE model group and puerarin treatment group at 12 h was 137.00 ± 5.00 and 78.20 ±4.38 respectively and that at 24 h was 164.40 ± 5.50 and 90.40 ±3.36 respectively, which was both significant higher than that in sham operated group （29.60 ±3.20 and 30.00 ± 3.74）. The expression of Bim protein in puerarin treatment group was lower than that in HIE model group. The ratio of viable apoptotic cell in cerebral cortex in HIE model group and puerarin treatment group at 12 h was （16.2±1.86） % and （8.54±1.91） % respectively and that at 24 hwas （20.03±2.02） % and （13.36±1.64） 96 respectively, which was both significant higher than that in sham operated group （（0.76±0.09） % and （ 1.34 ± 0.24） % ）, respectively. The ratio of viable apoptotic cell in puerarin treatment group was lower than that in HIE model group. Conclusion Puerarin can inhibit the expression of brain cell apoptosis initiator Bim protein in HIE neonatal Rats and has the action of resisting brain cell apoptosis.