人G-CSF基因组基因的克隆和序列测定

Human G-CSF Genomic DNA Cloning and its Sequence Analysis

用 PCR方法 ,以提取的正常中国人染色体 DNA为模板 ,成功地扩增出 1 .5kb的人G- CSF基因组基因。将其克隆至 p UC1 9,构建成重组质粒 p GG- 2。在酶切证实正确的基础上 ,将其酶切成三个片段进行了亚克隆。利用 p UC1 9的正反通用引物进行序列分析。自动测序结果表明其编码区的序列是正确的。

Chinese genomic DNA was used as template of PCR,1.5kb G CSF genomic DNA was successfully obtained by using PCR amplification method,and cloned to pUC19 plasmid.Sequence analysis showed that genomic DNA sequence of coding G CSF protein was correctly.