机会致病寄生原虫的系列研究

SERIAL STUDIES ON OPPORTUNISTIC PATHOGENETIC PARASITIC PROTOZOAN

本课题包括：①对机会致病寄生虫，包括弓形虫、卡氏肺孢子虫及隐孢子虫动物模型的建立，虫体的分离及提纯；②弓形虫感染的循环抗原（ＣＡ）和抗体（ＩｇＧ、ＩｇＭ），用竞争抑制酶联免疫吸附试验（ＩＥＬＩＳＡ）、金葡菌Ａ蛋白酶联免疫吸附试验（ＳＰＡＥＬＩＳＡ）、直接捕获法酶联免疫吸附试验（ＤＥＬＩＳＡ）进行检测；③用地高辛标记探针及聚合酶链反应（ＰＣＲ）检测核酸，自由引物ＰＣＲ鉴别弓形虫株以及生物特性；④用流式细胞仪对卡氏肺孢子虫及弓形虫的ＤＮＡ、ＲＮＡ及蛋白质含量的动力学进行了分析；⑤用激光微束系统对含８个子孢子成熟包囊施行手术，切除４个或２个子孢子后，包囊仍具有感染力。

The contents of serial studies involve isolation,purification and animal experimental model built up of opportunistic pathogenetic parasitic protozoan including Pnemocystis,Cryptosporidium and Toxoplasma; detection of circulating antigen (CA) and antibody (IgG,IgM) by I ELISA,SPA ELISA,D ELISA; determination of DNA by PCR and digoxigenin labeled probe; identification of Toxoplasma  strain by RP PCR and biological characters. Dynamics of Pneumocystis and Toxoplasma DNA,RNA and protein contents were analysed by Laser Flow Cytometry. Microsurgery on P. carinii  was made with ultrashort pulse laser microbeam system. Cysts being cut off 4 or 2 intrabodies had the ability to re infect made. It suggested that cyst with 8 intrabodies was mature one, etc.