淡水文石珍珠可溶性有机质对CaCO_3结晶的影响

The effect of soluble matrix proteins from aragonite pearls on the crystallization of CaCO_3

以浙江诸暨淡水文石珍珠为实验原料,提取文石珍珠中的两种基质蛋白——酸可溶蛋白(ASM)和水可溶蛋白(WSM),并分别将其制备成一系列不同浓度的基质蛋白溶液。利用上述两种不同浓度的可溶性有机基质溶液采用扩散法开展了碳酸钙的体外模拟矿化实验,探讨两种可溶性有机质的浓度在控制CaCO3结晶方面的作用。通过扫描电子显微镜及显微共聚焦拉曼光谱等方法对体外模拟矿化结果进行了观察和分析。结果表明,酸可溶蛋白对碳酸钙晶体的形貌和尺寸具有明显的调控作用,而对晶型无影响,不同浓度的酸可溶蛋白对碳酸钙晶体形貌影响差别不大,对尺寸的影响略有不同;水可溶蛋白对碳酸钙晶体的晶型、形貌及尺寸均有显著影响。不同浓度的水可溶蛋白对碳酸钙晶体的形貌和尺寸影响较大,对晶型有一定的调控作用。

Freshwater pearls （aragonite pearls） were selected as the main experimental materials to study the biomineralization of calcium carbonate. Two kinds of soluble matrix proteins,i.e.,acid soluble protein （ASM） and water soluble protein （WSM）,were extracted from aragonite pearls to prepare a series of aqueous solutions with different concentrations. In vitro study was carried out by using the above-mentioned aqueous solutions with different concentrations in the diffusion method. The effects of concentrations of ASM and WSM aqueous solutions were discussed in the controlled crystallization of calcium carbonate. Scanning electron microscopy （SEM） and Raman spectroscopy were used to observe and analyze the results of in vitro biomineralization of calcium carbonate. The results show some differences in regulation and controlled crystallization of CaCO3 between ASM and WSM aqueous solutions. The concentration of ASM aqueous solutions seems to play an important role in controlling the crystal morphology and size of calcium carbonate but it has no effect on polymorphism,while the polymorph,morphology and size of crystals are obviously influenced by the concentration of WSM aqueous solutions.