毛细管气相色谱法测定莪术油中β-榄香烯、莪术醇、吉马酮和莪术二酮的含量

GC determination of β-element,curcumol,germacrone and curdione of zedoary turmeric oil

目的:建立以毛细管气相色谱法测定莪术油中β-榄香烯、莪术醇、吉马酮和莪术二酮含量的方法。方法:采用气相色谱方法:HP-5(0.32 mm×30 m)石英弹性毛细管柱,进样口温度250℃,氢火焰检测器(FID)280℃,进样量2μL,不分流进样,柱流量1 mL.min-1;程序升温:柱初始温度为100℃,以4℃.min-1升至200℃。采用内标法,以水杨酸甲酯为内标物。结果:β-榄香烯进样量在0.03～0.49μg内与峰面积比值(β-榄香烯/水杨酸甲酯)呈良好的线性关系Y=5.024X-0.0815,r=0.9992,平均回收率98.6%(RSD为2.0%);莪术醇进样量在0.03～0.52μg内与峰面积比值(莪术醇/水杨酸甲酯)呈良好的线性关系Y=4.383X-0.0274,r=0.9993,平均回收率98.0%(RSD为2.5%);吉马酮进样量在0.14～2.20μg内与峰面积比值(吉马酮/水杨酸甲酯)呈良好的线性关系Y=0.969X-0.012,r=0.9997,平均回收率101.0%(RSD为2.3%);莪术二酮进样量在0.13～2.08μg内与峰面积比值(莪术二酮/水杨酸甲酯)呈良好的线性关系Y=0.9047X+0.0268,r=0.9994,平均回收率99.5%(RSD为2.5%)。结论:不同来源的莪术油中4种成分的含量差异显著,莪术二酮在部分样品中未检出。

Objective：To establish the method of determine the content of β-element,curcumol,germacrone and curdione in Zedoary turmeric oil by GC. Method：Gas chromatography： HP-5 （0.32mm × 30m） flexible quartz capillary column, injection port temperature 250 ℃, hydrogen flame detector （FID） 280 ℃, injection volume 2μL, do not split injection, column flow of 1mL · min^-1; programmed temperature： the initial column temperature of 100 ℃, with 4 ℃· min^-1 up to 200 ℃. methyl salicylate was adopted as internal standard. Result： β-elemene injection volume at 0.03― 0.49μg with the peak area ratio （β-elemene / methyl salicylate） was a good linear relationship Y = 5.0242X-0.0815, r = 0.9992, The average recovery rate of 98.6% （RSD 2.0%）; curcumol injection volume at 0.03― 0.52μg with peak area ratio （curcumol / methyl salicylate） was a good linear relationship Y = 4.3829X-0.0274, r = 0.9993, average recovery rate of 98.0% （RSD 2.5%）; Gemmaone injection volume at 0.14 ― 2.20μg with the peak area ratio （Germacrone / methyl salicylate） was a good linear relationship Y = 0.969X-0.012, r = 0.9997, average recovery rate of 101.0% （RSD 2.3%）; curdione injection volume at 0.13 ~ 2.08μg with peak area ratio （curdione / methyl salicylate） was a good linear relationship Y = 0.9047X ＋0.0268, r = 0.9994, average recovery rate of 99.5% （RSD 2.5%）. Conclusion： Different sources of zedoary 4 ingredients difference, curdione in some samples undetected.