HPLC法测定阿咖片中2种成分的含量

HPLC determination of two constituents in aspirin and caffeine tablets

目的:建立同时测定阿咖片中2种成分(阿司匹林、咖啡因)的HPLC法。方法:色谱柱为Phenomenex C18柱(4.6 mm×250 mm,5μm),流动相为1%醋酸-甲醇(55∶45),流速为1.0 mL.min-1,检测波长为272 nm,柱温为30℃。结果:阿司匹林和咖啡因的进样量分别在0.4864～5.8368,0.0564～0.6768μg范围内与峰面积呈良好线性关系,r均为1.0000(n=5),平均加样回收率分别为99.6%,100.2%;RSD分别为1.3%,0.9%(n=9)。结论:本方法快速简便,准确可靠。

Objective： To establish an HPLC method for determination of two constituents（aspirin and caffeine）in aspirin and caffeine tablets. Method： The analytical column was Phenomenex C18（4.6mm ×250mm, 5μm）.The mobile phase was 1% acetic acid -methanol （55：45） at flow rate of 1.0 mL·min^-1. Detection wavelength was set at 272nm and the column temperature was 30℃.Results： The linear ranges of aspirin and caffeine were 0.4864 -5.8368μg （r=1.0000 ,n=5） and 0. 0564～0.6768μg （r= 1.0000, n=5）,respectively. The average recoveries （n=9）of aspirin and caffeine were 99.6 %（RSD = 1.3%）,and 100.2%（RSD = 0.9%）, Conclusion：The method is simple ,accurate ,and suitable for determination of the three constituents in aspirin and caffeine tablets