肝癌中Transgelin表达与其预后相关

Expression of Transgelin in hepatocellular carcinoma is correlated with its prognosis

目的研究肝癌中Transgelin表达水平与肝癌复发及预后的关系。方法应用Western-blotting技术检测Transgelin在不同转移潜能肝癌细胞系中的表达，以及在临床肝癌手术标本中肝癌、癌旁组织及正常肝组织中的表达，并用免疫组织化学染色验证上述手术标本中Transgelin的表达水平。以Logrank检验及Kaplan-Meier分析其表达水平与肝癌病人总体生存和无瘤生存的关系。结果Western—blot法显示Transgelin在肝癌高转移潜能细胞系中的表达明显高于低转移潜能细胞系，其在肝癌组织中的表达也明显高于癌旁及正常肝组织，免疫组化结果与之相类似。Transgelin强阳性表达的病人术后生存率和无瘤生存率明显低于阴性表达者（P《0．01）。结论Transgelin与肝癌转移能力相关，可作为判断预后的指标。

Objective To investigate the correlation of the level of Transgelin expression in beptacellular carcinoma with its recurrence and prognosis. Methods The expression of Transgelin in different metastatic potential hepatocellular carcinoma cell lines and cancerous lesions, tissue adjacent to cancer lesions and normal liver tissues collected during the operation were detected by Western-blotting. The level of Transgelin expression in these clinical samples was accessed by immunohistochemical staining. The correlation between the level of Transgelin expression and the prognosis was analyzed by means of log-rank test and Kaplan Meier analysis. Results Western blotting test showed that the expression level of Transgeiin was significantly higher in high metastatic potential HCC cell line than in low metastatic potential cell line. Meanwhile, it was higher in tumor tissue than in tissue adjacent to cancer lesions or normal liver tissue. Immunohistocbemical staining test showed the similar result. Patients with positive expression of Transgelin had worse survival and tumor free survival than those with negative ones （P〈0.01）. Conclusion The expression of Transgelin in hepatocellular carcinoma is correlated with its metastatic potential and this protein could be the biomarker of the prognosis.