正畸力作用下大鼠白细胞介素-6的表达及牙槽骨改建的研究

The effects of orthodontic force on the expression of IL-6 and the alveolar bone remodeling in rats

目的:观察正畸力作用下IL-6在大鼠牙周组织内的表达分布及牙槽骨高度的改变,研究正畸力对牙周组织改建的作用。方法:将30只SD大鼠随机分为空白对照组和加力组,施加0.49 N近中向正畸力于加力组25只大鼠的左侧上颌第一磨牙。运用免疫组织化学及组织形态分析法观测各组大鼠上颌第一磨牙加力后0、1、3、5、7、10 d的IL-6表达量及牙槽骨吸收量。结果:加力组大鼠牙周组织内IL-6的表达在受力后第3天达到高峰,之后开始下降;其近中牙槽骨未见明显丧失。结论:正畸力虽可引发局部牙周组织的炎症反应及IL-6等促炎性细胞因子的释放,但具有一定的自限性,不会导致牙周组织的严重破坏和牙槽骨的明显吸收。

Objective： To investigate the expression and distribution of IL-6 in periodontal tissues and the change of the alveolar bone of rats during orthodontic tooth movement, and to study the effects of orthodontic force on the periodontal tissue remodeling. Methods： 50 gram orthodontic force was loaded on the left first maxillary molars of 25 rats in experimental group, lmmunohistochemistry and histomurphometrie analysis were performed to measure the expression of IL-6 and the loss of alveolar bone at 0, 1,3, 5,7 and 10 clays after the application of orthodontic force. Results： The expression of IL-6 was observed to reach maximum level on day 3 and to decline thereafter in experimental group. No obvious alveolar bone loss was detected in the mesial side of the first molars. Conclusion： Although orthodontic force can evoke the local inflammatory response of periodontal tissue and the expression of pro-inflammation cytokines such as IL-6, it can not cause severe periodontal destruction and alveolar bone loss.