镍铬合金与金铂合金烤瓷熔附金属全冠对犬牙龈组织的形态学影响

Influence of nickel-chromium alloy and gold-platinum alloy PFM on canine gingival tissues

目的:比较镍铬合金和金铂合金2种烤瓷熔附金属全冠(porcelain fused to metal,PFM)对犬牙龈组织结构的影响,观察PFM修复术的生物学效果,为临床上选择烤瓷熔附金属材料提供参考。方法:将3只健康犬以镍铬合金和金铂合金2种PFM修复犬牙,通过光镜和透射电镜观察犬牙龈组织的结构,用TUNEL方法检测细胞凋亡,并进行统计学分析。结果:镍铬合金PFM修复犬的牙龈组织,光镜下可见大量的炎性细胞浸润,局部血管出血;电镜下有大量细胞凋亡,凋亡指数为58.63%±11.12%,有细胞核固缩等反应。金铂合金PFM修复犬的牙龈组织光镜下未见大量的炎性细胞浸润及局部血管出血;电镜下少有细胞凋亡;凋亡指数为(26.90±17.35)%。统计分析显示镍铬合金PFM组和金铂合金PFM组的凋亡数与对照组都具有统计学意义,镍铬合金PFM组和金铂合金PFM组细胞凋亡数量有明显差异(P<0.05),镍铬合金组凋亡指数高。结论:镍铬合金PFM对犬牙龈组织结构的影响大,而金铂合金PFM对犬牙龈组织结构的影响小。临床PFM修复时,尽可能选择金铂合金制作PFM基底冠。

Objective： To compare the influence of nickel-chromium alloy and gold-platinum alloy porcelain-fused-to-metal （ PFM ） on uhramicrostructure of canine gingival tissues, to survey the biological effects after PFM full crown restoration, and to provide data for selection of PFM materials. Methods： The nickel-chromium alloy and gold-platinum alloy PFM full crowns were used to repair canines in 3 healthy dogs. The microstructure of canine gingival tissue was observed through light microscope and transmission electron microscope. Apoptosis of canine gingival cells was detected by TUNEL method. Results： Under light microscope, inflammatory cell infiltration and partial bleeding were seen in the nickel-chromium alloy PFM full crown repaired canine gingival tissues. Under transmission electron microscope, large amount of cell apoptosis and neuclear condensation were observed in these tissues,and apoptotic index was 58.63% ± 11.12%. While under light microscope, inflammatory cell infiltration and partial bleeding were not seen in the gold-platinum metal PFM full crown repaired canine gingival tissues. Under transmission electron microscope, only small amount of cell apoptosis was detected in these tissues,and apoptotic index was 26.90% ± 17.35%. Statistical significance was found in both nickel-chromium alloy group and gold-platinum metal group compared to control group. There was also statistical significance between nickel-chromium alloy group and gold-platinum metal group in cell apoptosis numbers （P 〈 0. 05 ）. Cell apoptosis number in nickel-chromium alloy group was much higher than that in gold-platinum metal group. Conclusion： The negtive effects on uhramicrostructure of gingival tissue using nickel-chromium alloy PFM are more evident than using gold-platinum metal. When select PFM to repair defect teeth, it is better to select the inactive gold-platinum metal PFM as base crown.